First Report of Deep Crypt Secretory Cells in Ascending Colon in Chinchilla (Chinchilla lanigera): A Histological and Histochemical Study

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Ghiurco Florin; Damian Aurel; Martonos Cristian; Matei Maria-Catalina; Latiu Calin; Rus Vasile; Miclaus Viorel & Gal Adrian Florin


The normal morphology of the colon differs among mammal species.The ascending colon presents several types of cells, responsible for carrying different functions for this organ. Among them, the mucus-secreting cells ensure the integrity of the mucosa, local defense, protection against different external factors, inflammatory diseases, cancer, etc. The ascending colon from 5 adult male chinchillas were processed for paraffin embedding and stained with three methods: Goldner’s trichrome, PAS reaction, and Alcian blue staining procedure. The results showed that the structure of the ascending colon is similar to the one described in other species, i.e. mucosa, submucosa, muscularis externa, and serosa. Regarding the mucus-secreting cells present in the deeper part of the mucosal crypts (deep crypt secretory or DCS cells) turned out to be different not only morphologically from the surface goblet cells but also regarding the type of mucus synthesized. DCS cells have a multivacuolated, faintly stained cytoplasm with moderately PAS-positive reaction and intensely positive reaction to Alcian blue stain. The mean surface of DCS cells was 521.6 μm2 as compared to 437.9 μm2 for goblet cells (p<0.05). In conclusion, our study describes for the first time in chinchilla (Chinchilla lanigera) the presence of formerly known non-goblet or vacuolated cells, and recently entitled DCS cells in the glandular epithelium of the colon. The understanding of morphological peculiarities in chinchi- lla may serve as a good basis to understand the pathophysiology of various conditions that may arise.

KEY WORDS: Chinchilla; Colon; DCS cells; Goblet cell; Mucous substances.

How to cite this article

GHIURCO, F.; DAMIAN, A.; MARTONOS, C.; MATEI, M. C.; LATIU, C.; RUS, V.; MICLAUS, V. & GAL, A. F. First reportof deep crypt secretory cells in ascending colon in chinchilla (Chinchilla lanigera): A histological and histochemical study. Int. J. Morphol., 39(4):1001-1005, 2021.