Immunohistochemical Localization of Vascular Factors in Tooth Germ of Amphibian (Cynops pyrrhogaster)

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Yoko Miwa ; Kingo Suzuki & Masataka Sunohara


Vascular endothelial growth factor (VEGF) and its receptor, VEGFR-2, are known to regulate blood vessel endothelium growth. They play important role in human and rodents teeth development. In newt jaws, there are sequential developmental teeth germs following behind the mature teeth. We examined the immunohistochemical localization of VEGF and its receptor and showed the specific expression pattern of VEGF and VEGF receptor in Cynops pyrrhogaster sequential tooth development. The intensity of immunoreactivity for VEGF in the inner enamel epithelium was weaker than that in the outer enamel epithelium in the dentine matrix formation and mineralization stages. Finally, at the enameloid maturation and enamel-like matrix formation stage, immunoreactivity for VEGF in inner enamel epithelium was stronger than in the outer enamel epithelium. The intensity of immunoreactivity for VEGFR-2 was positive for the outer enamel epithelium throughout tooth development. The crown sides of the odontoblasts were stained especially strongly for VEGF and VEGFR-2 during the dentine matrix formation and mineralization stage of the enameloid maturation and enamel- like matrix formation stage. We postulate that the expression of VEGF in the inner enamel epithelium and odontoblast widely effects tooth development in newts, as well as in human and rodents.

KEY WORDS: Vascular endothelial growth factor; Amphibian; Tooth germ.

How to cite this article

MIWA, Y. ; SUZUKI, K. & SUNOHARA, M. Immunohistochemical localization of vascular factors in tooth germ of amphibian (Cynops pyrrhogaster). Int. J. Morphol., 39(2):625-629, 2021.