High-Fat Diet Augments Ovariectomy-Induced Bone Resorption in Rats

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Mahmoud H. El-Bidawy; Nermeen Bastawy; Mohamed Mansour Khalifa; Hanan Abdulla; Rania Elsayed; Laila Rashed; Marwa Niazy; Sameer Al-Ghamdi & Mohamed A Haidara


Menopause complications such as cardiovascular and bone diseases represent a major public health concern. We sought to determine whether a high-fat diet (HFD) can augment ovariectomy-induced bone resorption in a rat model of menopause possibly via the upregulation of the inflammatory biomarkers and dyslipidemia. Rats were either ovariectomized and fed a standard laboratory chow (model group) or were ovariectomized and fed with a HFD for 15 weeks before being sacrificed. Ovariectomy significantly (p<0.05) increased body weight, dyslipidemia, insulin resistance, pro-inflammatory cytokines tumor necrosis factor-a (TNF-α) and interleukin-6 (IL-6), and biomarker of bone resorption, nuclear factor-kB (NF-kB), which were augmented by feeding animals with a HFD. This was confirmed through immunohistochemical study, where ovariectomy induced expression of p65/NF-kB protein in tibia bone sections of the model group, which were augmented by HFD. HFD augments ovariectomy-induced bone resorption through increased inflammatory biomarkers and NF-kB in rats.

KEY WORDS: Ovariectomy; Menopause; Inflammation; NF-kB, Dyslipidemia; Rat model.

How to cite this article

EL-BIDAWY, M. H.; BASTAWY, N.; MANSOUR, M. K.; ABDULLA, H.; ELSAYED, R.; RASHED, L.; NIAZY, M.; ALGHAMDI, S. & HAIDARA, M. A. High-fat diet augments ovariectomy-induced bone resorption in rats. Int. J. Morphol., 38(1):129- 134, 2020.