A Comparative Study of the Biocompatibility of Two Root-End Filling Materials in Rat Connective Tissue

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Francisco Javier Gutiérrez Cantú; Guadalupe Magaña Zamora; Ricardo Oliva Rodríguez; José Obed García Cortes; Wulfrano Sánchez Meraz & Jairo Mariel Cárdenas


The aim of the present study was to examine the short-term biocompatibility of Endosequence Root Repair Mate- rial (ERRM) paste and white Mineral Trioxide Aggregate MTA by implanting them into polyethylene tubes in the subcutaneous connective tissue of rats. twenty five male Wistar rats, 3-4 months old, weighing 300-350 g, were used. The tubes were implanted dorsally into the subcutaneous connective tissues of the rats. Five animals were sacrificed at five examination time points: 1, 3, 5, 7 and 15 days. The connective tissues containing the implants were excised. These sections were studied qualitatively and quantitatively using a light microscope. An average value for each group was obtained by averaging the sum of all inflammatory cells counted in 10 randomly selected, separate areas. For the ERRM group: There was a significant increase in the number of inflammatory cells on days 1-3 and on days 5-7 (P ≤ 0.003 and P ≤ 0.024). In the WHITE MTA group, the mean values of the sum of the inflammatory cells during the periods 1-3 days and 5-7 days were statistically significant (P ≤ 0.001 and P ≤ 0.044, respectively) and the XILOPERCHA group: Difference was observed significant in the value of the sum of inflammatory cells during the period of 3-5 days (P ≤ 0.05). According to the results it can be concluded that both, ERRM as MTA, caused an inflammatory reaction, which decreased over time; suggesting that both materials are biocompatible; showing however the presence of a higher organization of collagen fibers around the implants of ERRM.

KEY WORDS: Restorative materials; White MTA; Biocompatibility; ERRM.

How to cite this article

GUTIÉRREZ, C. F. J.; MAGAÑA, Z. G.; OLIVA, R. R.; GARCÍA, C. J. O.; SÁNCHEZ, M. W. & MARIEL, C. J. A comparative study of the biocompatibility of two root-end filling materials in rat connective tissue. Int. J. Morphol., 37(3):792-799, 2019.