Chemopreventive Effect of Brazilian Green Propolis on Experimental Dermal Carcinogenesis in Murine Model

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Rose Nely Pereira-Filho; Fellipe Santos Batista; Danielle Rodrigues Ribeiro; Genecy Calado de Melo; Francisco Prado Reis; Allan Ulisses Carvalho de Melo; Margarete Zanardo Gomes; Juliana Cordeiro Cardoso & Ricardo Luiz Cavalcanti de Albuquerque Júnior


The aim of this study was to assess the effect of oral administration of Hydroalcoholic Extract of Green Propolis (HEGP) on dermal carcinogenesis in rodent model. For the biological assay, we used 36 mice, assigned into 6 groups (n=6): CTR (treated with 100 mg/kg HEGP and no tumor induction), TUM (treated with water and tumor induction), GP10 (treated with 10 mg/kg HEGP and tumor induction), GP50 (treated with 50 mg/kg HEGP and tumor induction) and GP100 (treated with 100 mg/kg HEGP and tumor induction). Cancer induction was performed in the back of the mice by topical application of DMBA. After 16 weeks, mice were euthanized and their backs were submitted to post-mortem histological analysis. The mean number of lesions developed in TUM (4.14±0.89) was significantly higher than in GP10 (2.05±1.02), GP50 (1.8±1.92) and GP100 (2.5±1.73) (p<0.05). The tumors formed in HEGP-treated groups were histologically more differentiated, but only in PV100 in situ lesions were evidenced. Infiltration of anatomical noble structures was less frequent in HEGP-treated groups (p<0.05). Our data suggest that oral administration of HEGP provided partial inhibition of DMBA-induced dermal carcinogenesis, as well as appeared to modulate the differentiation and infiltrative potential of the carcinomas in rodent model.

KEY WORDS: DMBA; Propolis; Skin cancer.

How to cite this article

PEREIRA-FILHO, R. N.; BATISTA, F. S.; RIBEIRO, D. R.; MELO, G. C.; REIS, F. P.; MELO, A. U. C.; GOMES, M. Z.; CARDOSO, J. C. & ALBUQUERQUE-JÚNIOR, R. L. C. Chemopreventive effect of Brazilian green propolis on experimental dermal carcinogenesis in murine model. Int. J. Morphol., 32(2):522-530, 2014.