SUMMARY: Trachyspermum ammi (T. ammi) has been used in folk medicine as anti-inflammatory, antipyretic, antibacterial, anti- fungal agent. The present study was conducted to investigate the protective effect of Trachyspermum ammi (T. ammi) essential oil against CC1 kg/ip, negative control received CC1 1 mg/kg/ip + 0.5 mL distilled water orally and tree treatment groups which received CC1 similar to 44 the negative control and 200, 800 and 1600 μg/kg of T. ammi essential oil, respectively. All treatments were done twice a week (Saturday and Wednesday) for 45 days. On the last day, blood was sampled for urea and creatinine assessment and the left kidney was removed for stereological estimations. Essential oil of T. ammi at high dose significantly (p ≤ 0.05) decreased serum levels of creatinine and urea in comparison with CC1 -treated group. Total volume of the kidney, cortex, proximal convoluted tubules (PC), glomerulus, vessels and 4 interstitial tissue as well as total length of PC and vessel were significantly (p ≤ 0.05) increased following CC14 administration and were restored toward normal levels at high dose of T. ammi. Also, high dose of T. ammi improved glomerular loss significantly (p ≤ 0.05) as compared with CC14-treated group. Due to the chemical composition of T. ammi essential oil such as tymol, p-cymene,γ-terpinene which 4 induced nephrotoxicity in mice. Thirty-five mice were divided into five groups as follows; positive control received olive oil 1 mL/ are antioxidant, it can be concluded that the essential oil of T. ammi can ameliorated renal injury induced following CC1 antioxidant components.