Evaluation of Decellularized Matrix and β-Tricalcium Phosphate as Biomaterials for Bone Neoformation. In vivo Study

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Abraham Muñoz-Ruíz; Erika Silva-Benítez; Eduardo Soto-Sainz; Bernardino Cerda-Cristerna; Mariné Ortiz-Magdaleno; Amaury Pozos-Guillen & Héctor Flores


The aim of this study was to evaluate histologically the effect of two biomaterials, a biomaterial derived from porcine Urinary submucosa Bladder Matrix (UBM) and beta-TriCalcium Phosphate (β-TCP), on bone defects. Twenty male New Zealand rabbits were used; the models were divided in two groups: the UBM group; the β-TCP group, and a Negative Control (NC) group. Five- mm defects were created in the femur of each model and then the different biomaterials were set in place depending on each group. At 4 and 8 weeks, the animals in the models were sacrificed and samples of the defect site were collected to perform a Hematoxylin and Eosin stain (H&E). Histologically, β-TCP group at 4 and 8 weeks presented neoformation of bone-like and cartilage-like tissue, with the presence of inflammatory infiltrate; at 4 and 8 weeks, the UBM group presented neoformation of bone-like and cartilage-like tissue with a low presence of inflammatory infiltrate, and the NC group presented the formation of connective tissue and, in a low proportion, neoformation of bone tissue and cartilage. Both biomaterials, UBM and β-TCP, exhibited the capacity to promote bone neoformation; however, the UBM-based biomaterial produced a better-organized tissue with a lower inflammatory response compared with the β-TCP group.

KEY WORDS: Urinary bladder matrix; Bone regeneration; Biomaterial; β-tricalcium phosphate.

How to cite this article

MUÑOZ-RUÍZ, A.; SILVA-BENÍTEZ, E.; SOTO-SAINZ, E.; CERDA-CRISTERNA, B.; ORTIZ-MAGDALENO, M.; POZOS- GUILLEN, A. & FLORES, H. Evaluation of decellularized matrix and β-tricalcium phosphate as biomaterials for bone neoformation. in vivo study. Int. J. Morphol., 35(3):804-811, 2017.