Protective Effect of Lepidium sativum Seed Extract on Histopathology and Morphology of Epididymis in Diabetic Rat Model

Tweet about this on TwitterShare on FacebookEmail this to someoneShare on Google+

Mehran Kamani; Elahe Seyed Hosseini; Hamed Haddad Kashani; Mohammad Ali Atlasi & Hossein Nikzad


Diabetes mellitus is a frequent and serious metabolic illness all over the world and plants have been a desirable source of medicine recently. Diabetes has unpleasant effect on male reproductive system and it may lead to male infertility. It causes erectile dysfunction and reduces ejaculate volume by affecting the health of small blood vessels and the small nerves that control ejaculation and also decreases libido by decreasing testosterone levels. Current study evaluated the possible protective efficiency of Lepidium sativum (Garden cress) seed extract on fasting blood sugar (FBS) and then assessed histopathological change of epididymis in streptozotocine (STZ)-induced diabetic rats. We randomly categorized 50 adult male Wistar rats into five groups (each 10 rats). Group 1 was control placebo group receiving only 0.1 ml normal saline via gastric gavages, Group 2 as control diabetic rats received an intraperitoneal (IP) injection of STZ 60 mg/kg body weight. Rats with FBS >250 mg/dl were considered as diabetic. Group 3 were diabetic rats receiving insulin in dose 3U/100 g body weight and Groups 4 and 5 were diabetic rats that received 0.1 cc of 200 and 400 mg/kg‚ ethanol extract of Lepidium sativum seed by gavages daily. One day after the last gavages, rats were anesthetized by chloroform. Epididymis duct was removed from abdomen and weighed with a digital scale. Afterwards, samples were putted in Bouin's solution for histological measurement. Administration of 200 and 400 mg/ml doses of Lepidium sativum seed extract increased epithelium height and decreased interstitial volume density and fibro muscular thickness significantly. Also, volume density of epithelium, fibro muscular, lumen and interstitial decreased significantly. Tubular and lumen diameter did not change significantly in different groups. It appears Lepidium sativum seed extract is a beneficial protective supplementary agent against adverse effects of diabetes on male reproductive system.

KEY WORDS: Diabetes; Epididymis; Lepidium sativum (garden cress); Streptozotocine; Insulin.

How to cite this article

KAMANI, M.; HOSSEINI, E. S.; KASHANI, H. H.; ATLASI, M. A. & NIKZAD, H. Protective effect of Lepidium sativum seed extract on histopathology and morphology of epididymis in diabetic rat model. Int. J. Morphol., 35(2):603-610, 2017.