Histological Description of Morphogenesis of the Gastroesophageal Mucosa of Gallus gallus domesticus (Linnaeus, 1758)

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Adriana Ventura; Aparecida Alves do Nascimento; Marcos Antônio José dos Santos; Danielle Alcântara Vieira-Lopes; Armando Sales & Nadja Lima Pinheiro


The ontogenesis of the gastroesophageal mucosa involves morphological alterations related to its structure and the function of each segment. The present study describes the histogenesis of the mucus-secreting epithelium and glands of the esophagus, gizzard, and proventriculus of the chicken (Gallus gallus), and identifies alterations in the secretion pattern of glycosaminoglycans (GAG’s). We analyzed 38 chicken embryos, processed the material collected following the histological routine, and then stained it with hematoxylin-eosin for the analysis of tissue structure and with Gomori's trichrome for the identification of conjunctive tissue and collagen fibers. We used the PAS histochemical technique for the analysis of neutral GAG’s and the AB pH 2.5 histochemical technique for the analysis of acid GAG’s. The embryos at late stage of development had the esophagus wall composed of four layers: mucosa, submucosa, muscularis, and serosa, whereas the proventriculus and the gizzard were composed of three layers: mucosa, muscularis, and serosa. In all three segments, we identified the superficial epithelium as mucus-secreting; in the esophagus this epithelium was mucus- secreting only at the initial development stages. The proventricular glands began to form at the initial development stages, whereas the tubular glands began to form in the gizzard just after the 15th day. The differences in the production of GAG’s in these regions of the digestive tract are related to development stages, functions, and physiological requirements of each segment, and to the gradual adjustment of the body to the post-hatching life.

KEY WORDS: Chicken embryos; Development; Glycosaminoglycans; Esophagus; Stomach.

How to cite this article

VENTURA, A.; DO NASCIMENTO, A. A.; DOS SANTOS, M. A. J.; VIEIRA-LOPES, D. A.; SALES, A. & PINHEIRO, N. L. Histological Description of Morphogenesis of the Gastroesophageal Mucosa of Gallus Gallus Domesticus (Linnaeus, 1758). Int. J. Morphol., 31(4):1331-1339, 2013.