Tribulus terrestris Hydroalcoholic Extract Administration Effects on Reproductive Parameters and Serum Level of Glucose in Diabetic Male Rats

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Ali Ghanbari; Mojtaba Moradi; Amir Raoofi; Mahsa Falahi & Sepideh Seydi


Usage of medicinal plants for the treatment of diseases is a remarkable strategy considered in both clinical and experimental studies. This study was conducted to evaluate the effect of Tribulus terrestris hydroalcoholic extract administration on the serum level of glucose and reproductive parameters in diabetic male rats. In this experimental study, 30 adult male Wistar rats (200–250 g) were divided into 5 groups (n= 6). Diabetes was induced by a single intraperitoneal injection of 50 mg/kg streptozotocin. Experimen- tal groups received daily Intraperitoneal injection of the extract (100, 250 and 500 mg/kg) since one week after inducing diabetes for two weeks. At the end of the experiment, the serum levels of testosterone and glucose were examined by ELISA and Glucodr devices respectively. Sperm parameters including morphology, count and motility were evaluated from the cauda epididymis. The diameters of seminiferous tubules were assessed based on 5 μm paraffin section of H&E stained section of testes. The data were evaluated by One Way ANOVA followed by post-hoc test using SPSS software. In T. terrestris extract groups, there were significant reductions in the levels of blood glucose while increased sperm motility, sperm count and seminiferous tubules diameter, percentage of sperms with normal morphology, level of testosterone hormone and final body weight compared with diabetic group (p <0.001). In conclusion, T. terrestris hydroalcoholic extract decreases serum level of glucose and diminishes the side effects of diabetes on male reproductive system showing by increasing in the serum level of testosterone, improving sperm parameters and returning the seminiferous tubules to normal diameter by a dose dependent manner.

KEY WORDS: Diabetes; Glucose; Rat; Streptozotocin; Testosterone; Tribulus terrestris.

How to cite this article

GHANBARI, A.; MORADI, M.; RAOOFI, A.; FALAHI, M. & SEYDI, S. Tribulus terrestris hydroalcoholic extract administration effects on reproductive parameters and serum level of glucose in diabetic male rats. Int. J. Morphol.,34(2):796-803, 2016.