Prenatal Stress Caused by Movement Restriction Induces Changes in the Appendicular Osseous Development of CF-1 Mice Progeny

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Pablo Lizana; Ricardo Henríquez & Pablo Muñoz


Prenatal stress has been associatedwith alterationsin weight andbody size, as well as disturbances inthe process ofdevelopingskeletalossification, occurringduring childbirth and the early stagesof life.However, theeffectevidence of prenatal stresson bone growthand development duringthe gestation periodhas been low; therefore, it is unknown whether these alterations are associated with potential for growth disorders Because of this, thestudy aims todetermine theshort-term effectsof prenatal stress onthe CF-1mouse bone structure growth inyour date of birth. The female mice were divided randomly in two groups: controlled (n=2) and stressed (n=3). The latter was put under stress by means of movement restriction during the last week of gestation. Second, an evaluation of their gestational development was made, obtaining measurements of their weight. Finally, diaphanization with KOH and staining with Alizarin red was used to measure the length of their appendicular bones and their flat pelvic bones, of 53 P0 mice (25 control; 28 stressed during gestation). The stressed mice's body weight (p=0.02) and the length of their appendicular bones (radii, p=0.0011; ulnae, p= 0.0001; humeri, p=0.0001; femorae, p=0.0006; tibiae, p=0.0015) were affected significantly in contrast with the controlled group. On the other hand,there were nosignificant differences inmaternal bodyweightand length ofthe mice pelvic bones (isquium, ilium; p>0.05). The prenatal stress by means of movement restriction alters the osseous appendicular morphology of the CF-1 mouse evaluated at birth.

KEY WORDS: Prenatal Stress; CF1 Mouse; Alizarin red; Appendicular Skeletal; Morphometric, Movement restriction.

How to cite this article

LIZANA, P.; HENRÍQUEZ, R. & MUÑOZ, P. Prenatal stress caused by movement restriction induces changes in the appendicular osseous development of CF-1 mice progeny. Int. J. Morphol., 30(3):1132-1138, 2012.