Immunohistochemical Analysis of Human Homologue of Drosophila Patched (PTCH) in Dental Follicles of Impacted Third Molars

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David Moraes de Oliveira; Márcia Maria Ferreira da Silveira; Emanuel Sávio de Souza Andrade; Ana Paula Veras Sobral; Paulo Ricardo Saquete Martins-Filho; Thiago de Santana Santos; Patrícia Leimig Amorim de Oliveira; Aline Carvalho Peixoto; Jadson Alípio Santana de Souza Santos & Marta Rabello Piva


This study investigated the immunodetection of PTCH in epithelial components of dental follicles associated with impacted third molars without radiographic signs of pathosis. One hundred and five specimens of dental follicles associated with impacted third molars with incomplete rhizogenesis (between Nolla's stage 6 and 9) were surgically removed from 56 patients. Epithelial cell proliferation was determined by using immunohistochemical labeling. Statistical analysis was performed using Fisher exact test and a level of significance of 5%. Of the 105 dental follicles collected, 3 were PTCH-positive. The specimens with squamous metaplasia and epithelial hyperplasia had higher rates of positivity for PTCH, as well as those with active remnants of odontogenic epithelium. This study suggests that the odontogenic cells of the dental follicle might be proliferating during the rhizogenesis, while the squamous metaplasia and hyperplasia of the epithelial lining and proliferative odontogenic epithelial rests show the differentiation potential of dental follicles.

KEY WORDS: Immunohistochemistry; Dental follicle; Odontogenic epithelial; PTCH.

How to cite this article

OLIVEIRA, D. M.; SIVEIRA, M. M. F.; ANDRADE, E. S. S.; SOBRAL, A. P. V.; MARTINS-FILHO, P. R. S.; SANTOS, T. S.; OLIVEIRA, P. L. A.; PEIXOTO, A. C.; SANTOS, J. A. S. S. & PIVA, M. R. Immunohistochemical analysis of human homologue of drosophila patched (PTCH) in dental follicles of impacted third molars. Int. J. Morphol., 30(1):105-109, 2012.