Bovine Bone Matrix Action Associated With Morphogenetic Protein in Bone Defects in Rats Submitted to Alcoholism

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Rogério Leone Buchaim; Jesus Carlos Andreo; Antonio de Castro Rodrigues; Daniela Vieira Buchaim; Domingos Donizeti Roque; José Sidney Roque & Geraldo Marco Rosa Júnior


Extended excessive alcohol use causes changes in bone tissue, thus affecting osteogenesis. The objective of this study was to evaluate if demineralized bone matrix (Gen-ox®) associated with bone morphogenetic protein (Gen-pro®) changes bone neoformation in rats submitted to experimental alcoholism. Forty male rats (Rattus norvegicus) were separated into 2 groups of 20 animals each: Group E1, which received ethyl alcohol at 25% and had the surgical cavity filled in only with blood clot; and Group E2, which received ethyl alcohol at 25% and had the surgical cavity filled in with demineralized bovine cortical bone associated with bone morphogenetic protein. The animals were submitted to a three-week period of gradual adaptation to alcohol, and then continued receiving alcohol at 25% for 90 days, when the surgical cavity was made. After the surgery, the animals continued consuming alcohol until reaching the sacrifice periods of 10, 20, 40, and 60 days, when the tibias were removed for histological processing. Results showed that surgical cavity repair and bone marrow reorganization occurred faster in Group E1 than in Group E2. At the end of the experiment, it was observed that animals in Group E2 had thick bony trabeculae surrounding the implanted material particles and a small area of connective tissue in the surface region. In conclusion, the implanted material did not accelerate bone neoformation, rather it served as a structure for osteogenesis.

KEY WORDS: Bone repair; Alcoholism; Xenograft; Bone morphogenetic proteins.

How to cite this article

BUCHAIM, R. L.; ANDREO, J. C.; RODRIGUES, A. C.; BUCHAIM, D. V.; ROQUE, D. D.; ROQUE, J. S. & ROSA-JÚNIOR, J. M. Bovine bone matrix action associated with morphogenetic protein in bone defects in rats submitted to alcoholism. Int. J. Morphol., 30(1):266-271, 2012.