Quantitative Evaluation of the Regenerated Dura Mater in a Decompressive Skull Trepanation Model in Rats

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Panteleichuk Andrii B.; Shmeleva Anna A.; Kadzhaya Mykola V.; Hnatiuk Olena P.; Karakhim Sergiy I.; Dovbeshko Galina I.; Savosko Serhii I. & Kozakevych Roman B.

 

Summary

Regeneration of the dura mater following duraplasty using a collagen film, a chitosan film, or a combination of both with gelatin, was studied in a craniotomy and penetrating brain injury model in rats. Collagen autofluorescence in the regenerated dura mater was evaluated using confocal microscopy with excitation at λem = 488 nm and λem = 543 nm. An increase in regeneration of the extracellular matrix of connective tissue and an increase in matrix fluorescence were detected at 6 weeks after duraplasty. The major contributors to dura mater regeneration were collagen films, chitosan plus gelatin-based films, and, to a much lesser extent, chitosan-based films. By using autofluorescence densitometry of extracellular matrix, the authors were able to quantify the degree of connective tissue regeneration in the dura mater following duraplasty.

KEY WORDS: Dura mater; Duraplasty; Fluorescence; Densitometry.

How to cite this article

PANTELEICHUK, A. B.; SHMELEVA, A. A.; KADZHAYA, M. V.; HNATIUK, O. P.; KARAKHIM, S. I.; DOVBESHKO, G. I.; SAVOSKO, S. I. & KOZAKEVYCH, R. B. Quantitative evaluation of the regenerated dura mater in a decompressive skull trepanation model in rats. Int. J. Morphol., 39(6):1731-1736, 2021.