Investigation of ADAMTS-1, BAX and IL-10 Expressions in Granulosa Cells Determinanting Fertilization Success in IVF

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Yunus Çavus & Ugur Deger


Granulosa cells (GCs) are essential components of follicles and play a role in regulating follicle development. The aim of this study was to investigate certain cellular components involved in the proliferation, differentiation and functional characteristics of granulosa cells in the success of fertilization of human oocytes during invitro fertilization (IVF) via immunohistochemical techniques. In this study, 30 patients who were diagnosed as primary or secondary infertility, polycystic ovary syndrome in the IVF center of Memorial Hospital, Department of Obstetrics and Gynecology were included. The amount of Anti Müllerian Hormone (AMH) in blood and granulosa cell diameter and cell core diameter were measured in 20 cells collected from each patient. In addition, degeneration scoring and BAX, ADAMTS-1, IL-10 expressions in granulosa cells were evaluated (p <0.01). It was thought that apoptosis induced by human GCs might be an indicator of egg quality. Moderate expression of ADAMTS-1 was thought to be related to failure of ovulation, deterioration of oocyte quality and decreased fertilization rate. This decrease in AMH levels may be associated with defects in granulosa cells. Therefore, significantly lower AMH secretion and increase in IL10 expression levels in healthy people can be explained by the increase of granulocyte cells.

KEY WORDS: Granulosa Cells: Fertility: ADAMTS-1; IL-10: BAX: immunohistochemistry.

How to cite this article

ÇAVUS, Y. & DEGER, U. Investigation of ADAMTS-1, BAX and IL-10 expressions in granulosa cell determinanting fertilization success in IVF. Int. J. Morphol., 38(2):427-434, 2020.