Anti-Angiogenic Effect of Caffeic Acid and Pinocembrin in the Process of Physiological Angiogenesis of Chicken Fetuses

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Patricia Olivares O.; María Paz Riquelme; Pedro Leiva A.; Luis A. Salazar; Dina Guzmán-Oyarzo & Carlos Rosas C.


Angiogenesis is the process by which new blood vessels are formed from other existing ones. A balance between proangiogenic factors and anti-angiogenic factors within the microenvironment must exist for the process to be carried out correctly. Similarly, the existence of natural chemicals such as polyphenols, which are capable of being acquired in the diet, induce these factors in the angiogenic process. Polyphenols were administered in the methylcellulose filters on the of chorioallantoic membrane of White Leghorn eggs, maintaining the normal posterior development of the fetus. 15 chicken fetuses were fixed in buffered formalin, obtaining the hearts to histological processing, performing histological, histochemical and immunohistochemical techniques. VEGF levels and the ability of the blood vessels growing under the stimulation of the polyphenols were evaluated. Immunoreactivity was quantified by Image J. The results indicate that caffeic acid and pinocembrin decreased microvascular density and VEGF expression in hearts stimulated with these polyphenols. Both the caffeic and pinocembrin acids play an inhibitory role in the physiological angiogenesis process in the chicken heart, which decrease the microvascular density and could act by modulating the signaling pathways mediated by the VEGFR or by modulating the availability of VEGF. The use of these polyphenols could be useful in studies of other tissues associated with pathological angiogenesis.

KEY WORDS: Angiogenesis; Poliphenols; Caffeic acid; Pinocembrin.

How to cite this article

OLIVARES, O. P.; RIQUELME, M. P.; LEIVA, A. P.; SALAZAR, L. A.; GUZMÁN-OYARZO, D. & ROSAS, C. C. Anti-angiogenic effect of caffeic acid and pinocembrin in the process of physiological angiogenesis of chicken fetuses. Int. J. Morphol., 38(1):135-139, 2020.