Effects of Underwater Lipus on the Morphohistology of the CF-1 Mice Growth Plate in Late Lactation Stage

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Ricardo Henríquez; Tuillang Yuing; Gonzalo Pallauta; Roberto Muñoz; Adolfo Saavedra; Paulina Nuñez & Mario Valenzuela


Ultrasound is one of the most used tools in physiotherapy, its LIPUS modality allows to treat musculoskeletal injuries, but like the conventional ultrasound it is contraindicated its application in areas close to growth cartilage by a possible closure of this one. In the literature there is no conclusive evidence of this contraindication, so the objective of the present study is to evaluate the effects of doses equivalent to underwater LIPUS on the histomorphometry of the proximal tibial epiphysis growth plate in an animal model. LIPUS was applied in underwater mode in the right pelvic limb to 8 mice in p 15 for 4 days, 2 times a day. On completion of 8 sessions the histology of the stimulated limb growth plate was sacrificed and the histology. When evaluating the histomorphometry of the plaque, a significant increase in the thickness and area of the proliferative zone stimulated with LIPUS (p = 0.048) was observed. In the hypertrophic area were observed no significant differences in thickness or areas, but in the hypertrophic area there were significant changes in the histological organization, observing a significant increase of the entire columns in the stimulated plaque (p = 0.04). LIPUS in underwater modality modifies the histomorphometry of the proximal epiphyseal PC of the tibia of rodents in the late postnatal stage, promoting proliferation and columnar organization in the proliferative and hypertrophic zone, respectively.

KEY WORDS: Low-intensity pulsed ultrasound; CF-1 mice; Late lactation; Growth Plate.

How to cite this article

HENRÍQUEZ, R.; YUING, T.; PALLAUTA, G.; MUÑOZ, R.; SAAVEDRA, A.; NUÑEZ, P. & VALENZUELA, M. Effects of underwater lipus on the morphohistology of the CF-1 mice growth plate in late lactation stage. Int. J. Morphol., 35(4):1351-1358, 2017.