Evaluation of Mesenchymal Stem Cell Proliferation Using Different Low Intensity Pulsed Ultrasound Intensities

DOI : http://dx.doi.org/10.4067/S0717-95022012000200043
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Daniela Becerra G.; Jaime Díaz Z.; Flavio Carrión A.; Carolina Inostroza S. & Rodrigo Oyonarte W.

Summary

Mesenchymal Stem Cells (MSCs) can be affected in their capabilities to proliferate in vitro under physical and/or biochemical stimulation. The aim of this study was to select an optimal intensity level for low intensity pulsed ultrasound (LIPUS) stimulation of Sprague-Dawley bone marrow MSCs proliferation in vitro. Bone marrow MSCs of Sprague-Dawley rats where cultured (a-MEM, 20%FBS and 1%antibiotic) and stimulated with LIPUS (0,02 milisec), for 20 minutes twice daily for 10 days, at intensities of 0 (control), 30 and 50mW/cm2 (groups A, B, C). Cellular count and morphological evaluation were performed. ANOVA and Bonferroni tests were performed. LIPUS-stimulated cultures displayed greater cellular counts, and significant differences were observed between groups A and C (p<0,05). Morphological differences were observed between cells from LIPUS-stimulated and control groups. An intensity of 50mW/cm2 elicits increased cellular proliferation (p<0.05). Stimulation of MSCs cultures with LIPUS influences cellular morphology.

KEY WORDS: Adult stem cells; Mesenchymal stem cells; ultrasound.

How to cite this article

BECERRA, G. A.; DÍAZ, Z. J.; CARRIÓN, A. F.; INOSTROZA, S. C. & OYONARTE, W. R. Evaluation of mesenchymal stem cell proliferation using different low intensity pulsed ultrasound intensities. Int. J. Morphol., 30(2):621-626, 2012.