Morphological Aspects of Spermatogenic Cells at Different Stages of Sexual Maturation in Black Isogenic C57BL/6/Uni Mice

DOI : http://dx.doi.org/10.4067/S0717-95022012000100052
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Martins, M. R. F. B.; Silva, P. J. R.; Martins,B. B.; Zoca, S. M.; Freitas, M. A. & Batista, T. C.

Summary

In order to study the morphological changes that occur in cells of the testes of isogenic black mouse C57BL/6/Uni into three periods during spermatogenetic used 15 mice divided into 3 groups of 5 animals with 40, 50 and 60 days of age. The mice were sacrificed and weighed. Then weighed and measured the testicles, to be processed finalmete histologically and stained with HE, PAS and Masson Massom-H and evaluated under light microscopy. Was observed in group I with 40 days of age in the seminiferous tubules had a lumen with sparse small amount of interstitial tubular cells. In the seminiferous epithelium were identified type A spermatogonia, intermediate and B, which occupied the compartment adbasal and intermingled with these cells was observed in spermatocytes I in Pachytene and leptotene, whereas in the adluminal compartment Golgi phase spermatids observed in the presence of acrosomal granule. In group II, the cells of the seminiferous epithelium were developed and it was observed in round spermatids cephalic hood phase plus many elongated spermatids in acrosome phase and Sertoli cells. In Group III, 60 days old, it was found that the seminiferous epithelium which was of the tubules had elongated spermatids in acrosome phase and maturation, with elongated nuclei and acrosomal system typical of spermiation in the presence of sperm and residual bodies near the tubular lumen. So you can check the morphological evolution of germ cell testicular spermatids and recognize its four phases: Golgi, cap, acrosome and maturation over the age of the animal.

KEY WORDS: Spermatogenesis; Mouse; Testes.

How to cite this article

MARTINS, M. R. F. B.; SILVA, P. J. R.; MARTINS, B. B.; ZOCA, S. M.; FREITAS, M. A. & BATISTA, T. C. Morphological aspects of spermatogenic cells at different stages of sexual maturation in black isogenic C57BL/6/Uni mice. Int. J. Morphol., 30(1):290- 295, 2012.