Effects of 14 weeks resistance training on muscle tissue in Wistar rats.

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Robson Chacon Castoldi; Giovana Rampazzo Teixeira; Olga Cristina de Mello Malheiro; Regina Celi Trindade Camargo; William Dias Belangero & José Carlos Silva Camargo Filho


The objective of the present study was to analyze the effects of 14 weeks of resistance training on muscle tissue in Wistar rats. 20 male Wistar rats were used in the study; 10 allocated to the untrained group (Group 1) and 10 to the trained (Group 2). The physical training (PT) program consisted of four sets of ten water jumps, three times a week for 14 weeks. A progressive increase in load was applied from the14nd to the 42nd day. Following this, the animals were euthanized with an overdose of potassium chlorate (100 mg/ kg) applied intraperitoneally. The ventral portion of the gastrocnemius muscle was removed for histological processing. The muscles were frozen in liquid nitrogen and cut into thicknesses of 5 μm using a rotative microtome. The sections were stained with hematoxylin-eosin for general analysis of the structure of muscles and analysis of the area of muscle fibers. The reaction with NADH-TR was utilized for differentiation between fast twitch (FT) and slow twitch (ST) fibers. The sections subjected to staining and histochemical reactions were observed in normal and polarized light and photomicrographed using a microscope (Zeiss®). The measurement of muscle fibers was performed using AxioVision software (Zeiss®). Statistical analysis was performed using the Student t test for analysis of the samples average. An average area of 5061.29 μm2 for Group 1 versus 5768.93 μm2 for Group 2 was observed. It can be concluded that 14 weeks of training with water jumps was effective in increasing muscle area. An increase in transverse area section (TAS) of ST and FT was also verified in the trained group.

KEY WORDS: Swimming; Striated muscle; Physical Exercise.

How to cite this article

CASTOLDI, R. C.; TEIXEIRA, G. R.; MALHEIRO, O. C. M.; CAMARGO, R. C. T.; BELANGERO, W. D. & CAMARGO FILHO, J. C. S. Effects of 14 weeks resistance training on muscle tissue in Wistar rats. Int. J. Morphol., 33(2):446-451, 2015.